Przemko Tylzanowski
Medical University of Lublin, Poland
University of Leuven, Belgium
przemko@kuleuven.be
DAY I (20.04.2017):
8.30-9.00 Registration
9.00-9.30 Welcome and Introduction to Practical Part
9.30-12.30 Practical part I – coordinator: Piotr Podlasz (University of Warmia and Mazury in Olsztyn) /Coffee break when needed
12.30-13.30 Sandwich lunch
13.30-14.15 Introduction to zebrafish - Przemko Tylzanowski (Medical University of Lublin /University of Leuven)
14.15-15.15 Legal aspects (Lecture in Polish)- Mirosław Łańcut (Medical University of Lublin)
15.15-15.45 Coffee break
15.45-16.30 Zebrafish husbandry, breeding and health – Małgorzata Wiweger (International Institute of Molecular and Cell Biology in Warsaw)
16.30- 17.15 Zebrafish model in toxicology - Camila Esguerra (Biotechnology Centre of Oslo, University of Oslo) (to be confirmed)
17.15-17.35 Anima IT (Techniplast)
17.35-17.55 Pros of lab husbandry of fish. -Tomasz Konicki Pentair/ Aquatic Habitats)
17.55 – 20.00 Poster session/ Wine & cheese party
DAY II (21.04.2017):
9.00-12.30 Practical part II (Coffee break when needed)
12.30-13.30 Lunch & Discussion
13.30-14.50 Practical part III (Coffee break when needed)
14.50-15.00 Final conclusions and departure
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 09.30– 10.30 | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) | Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) | Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot) | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) |
| 10.30- 11.30 | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) | Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) | Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot) |
| 11.30-12.30 | Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot) | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) | Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) |
| 12.30-13.30 | Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) | Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot) | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) |
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 9.00-9.50 | Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk) | Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica) | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) | Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) |
| 9.50-10.40 | Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) | Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk) | Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica) | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) |
| 10.40-11.30 | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) | Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) | Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk) | Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica) |
| 11.30-12.20 | Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica) | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) | Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman) | Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk) |
| 12.30-13.30 | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion |
| 13.30-13.50 | Time for repetition of - selected issues | Chemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec) | Time for repetition of - selected issues | Dissection of organs from adult zebrafish (Anna Jakimiuk) |
| 13.30-13.50 | Dissection of organs from adult zebrafish (Anna Jakimiuk) | Time for repetition of - selected issues | Chemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec) | Time for repetition of - selected issues |
| 13.30-13.50 | Time for repetition of - selected issues | Dissection of organs from adult zebrafish (Anna Jakimiuk) | Time for repetition of - selected issues | Chemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec) |
| 13.30-13.50 | Chemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec) | Time for repetition of - selected issues | Dissection of organs from adult zebrafish (Anna Jakimiuk) | Time for repetition of - selected issues |
Przemko Tylzanowski
Medical University of Lublin, Poland
University of Leuven, Belgium
przemko@kuleuven.be
Mirosław Łańcut
Medical University of Lublin, Poland
miroslawlancut@umlub.pl
Małgorzata Wiweger
International Institute of Molecular and Cell Biology
in Warsaw, Poland
mwiweger@iimcb.gov.pl
Camila Esguerra
Biotechnology Centre of Oslo, University of Oslo, Norway
c.v.esguerra@biotek.uio.no
Practical parts coordinator
Piotr Podlasz
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland
piotr.podlasz@uwm.edu.pl
Anna Jakimiuk
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland
annajakimiuk@wp.pl
Natalia Kasica-Jarosz
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn
natalia_kasica@wp.pl
Natalia Nowik
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland
natalia.nowik@uwm.edu.pl
Institute of Biology, Leiden University, The Netherlands
n.nowik@umail.leidenuniv.nl
Aneta Chełstowska-Wejman
Eppendorf
Agnieszka Jakubaszek
Medical University of Lublin, Poland
Anna Sobolewska
Medical University of Lublin, Poland
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