Offline Website Builder| Zebrafish housing system capacity (e.g. number of racks): | 3 rack (Tecniplast Multilink ZebTec System), 3 homemade rack |
|---|---|
| Current animal core facility/
or for laboratory purposes: | Facility for the needs of the University and others |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | 2 technicians |
| Services: | Fish maintaining, embryos collection Toxicity tests (FET, DTT, OSA), Behavioral tests, Microiniections Zebrafish xenograft model, Genotyping Study of the gene expression with qPCR, Professional guidance for establishing zebrafish facility for scientific or educational purposes. |
| Research conducted using the zebrafish model, grants: | ● Study of the function of galanin in physiological and pathological conditions using zebrafish as a model organism.
● Zebrafish xenograft model with cancer lines derived from domestic animals. Research on substances of plant origin in terms of anti-cancer properties. ● Exploring the potential of natural extracts for pharmaceutical applications, with special focus on inflammatory processes. ● Study of the function of the galanin during regeneration of the nervous system using zebrafish as a model organism ● Neuroscience, particularly in the field of neurodegenerative diseases, epilepsy and anxiety. ●Role of natural products in overcoming drug resistance in Mycobacterium tuberculosis |
| Specialized equipment: | DanioVision with EthoVision XT software (Noldus) LSM 700 Confocal Microscope (Zeiss) Fluoroimager Typhoon 9410 (GE Healthcare) Laser Speckle Contrast Imaging System (RWD) Microinjection system (InjectMan® NI 2, FemtoJet® express, Eppendorf) Micropipette Puller MP-500 (RWD) QuantStudio™ 3 Real-Time PCR System (Applied Biosystems™) |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains:
● AB, TU, Transgenics: ●Tg(mnx1:TagRFP) ● Tg(ins:NTR-mCherry) ● TgBAC(neurod1: EGFP) ● Tg(Fabp10a:DsRed)/ Tg(ela3l:EGFP) ● Tg(BRN3C:GFP) ● Tg (POMCA: GFP) ● TgBAC(prl:RFP) ● Tg(mpx:GFP) ● Tg(Fli1a:GFP) ● Tg(mpeg1:mCherry) ● TgBAC(gal:GAL4-VP16) ● Tg(UAS:Dendra-kras) ● TgBAC(chata:GAL4-VP16) ● Tg(HGn39D: GFP) ● Tg(hsp70l:adcyap1b)a140 ● Tg(hsp70l:galanin)a135 Mutants/Crispants ● Casper ● Galn -/- ● scn1labs552/s552 |
| Zebrafish housing system capacity (e.g. number of racks): | Tecniplast, 21 racks w/o quarantine |
|---|---|
| Current animal core facility/
or for laboratory purposes: | Zebrafish Core Facility (ZCF) |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | 4 FTE |
| Services: | Husbandry and breeding of D.rerio lines including weekends and holidays, animal welfare, health monitoring, setting spawners, sorting of transgenic lines, aquatic system assistance 24/7, fin-clipping and swabbing for the fish genotyping purpose, cryopreservation of lines including sperm freezing (SF) and in-vitro fertilization (IVF), importation/exportation of the lines, taking care of the equipment and consumables in the facility/laboratory/behavioral/injection room, practical training of new researchers, theoretical training platform for English researchers conducting procedures on animals. |
| Research conducted using the zebrafish model, grants: | Current NCN grants:
• The role of mTOR-Brg1 interaction in normal and aberrant neuronal activity. • Rac1 contribution to brain connectivity impairments and neuropsychiatric disorders in Tuberous Sclerosis Complex. • How inactivation of mitochondrial calcium uniporter protects dopaminergic neurons. • Experimental analysis of molecular determinants involved in epilepsy. • Building a genomic atlas of human inner ear malformations: focus on novel genes and functional non-coding regions. • Reconstructing cardiovascular cell lineage evolution, one cell at a time. • CRISPR tools for the study of embryonic development in zebrafish. |
| Specialized equipment: | • Complete workstations for zebrafish embryo’s micromanipulation enable injecting genetic material and creating new transgenic or knock-out lines (WPI and Femtojet pumps, Narishige micromanipulators, Leica stereoscopes).
• Needle puller, tips beveler, and microforge for the precise preparation of injection microcapillaries. • 1x Fluorescent stereomicroscope Leica that enables to observe and image of embryos/larvae in bright field, dark-field, or prepare fluorescent pictures. • Light-sheet fluorescent microscope Zeiss that provides high resolution in vivo and real-time imaging of zebrafish. • The behavioral testing room is equipped with a stand-alone aquatic rack (Aquaneering) for fish maintenance and automated systems for observing and tracking larval and adult zebrafish (Viewpoint Zebrabox, Zebracube). |
| Available zebrafish strains, transgenic lines, mutants: | WT: AB*, TL*, TU*, ABxTL
Transgenic: EGFP (twu34Tg), mnx1:TagRFP (vu504Tg), cmlc2:mRFP: (md3Tg), mito:EGFP (cms1Tg), fli:GFP (y1Tg), Vasa:EGFP (zf45Tg), Brn3c:GFP (s356tTg), myl7:gCaMP (s878Tg), hand2:GFP (pd24Tg), fabp10a: GCaMP5G)]*, ptf1a:EGFP (jh1Tg), ath5:mRFP (cu2Tg), nkx2.5 EGFP (el83Tg), DsRed (gz4Tg), cmlc2:EGFP (s883Tg), GCaMP5G [Tg(elavl3: myl7:) Mutants: tsc2 (vu242), MCU (sh214), pink1 (sh397), stim2b (waw301), stim2a (waw303), IT8 (waw302), Casper, Albino, Nacre * Signed Material Transfer Agreement (MTA) |
| Zebrafish housing system capacity (e.g. number of racks): | 6 regular racks, 1 genotyping rack |
|---|---|
| Current animal core facility/
or for laboratory purposes: | Zebrafish Core Facility |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | 3 |
| Services: | breeding fish |
| Research conducted using the zebrafish model, grants: | Core facility operates as a service centre for different researchers who perform toxicological, pharmacological, oncological, behavioural, neurological, cardiovascular, genetic and other studies. |
| Specialized equipment: | - behavioural research - Noldus, Zebrabox, Zebracube
- EEG set for zebrafish testing - Scientifica - MRI, system Bruker PharmaScan 7T with CryoProbe Complete list of research equipment is available at: https://omd.umlub.pl/laboratoria/wykaz-aparatury-badawczej/ https://omd.umlub.pl/laboratoria/galeria-aparatury-badawczej/ |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains:
● AB Transgenics: ● Tg(Fli1a:GFP) |
| Zebrafish housing system capacity (e.g. number of racks): | 1 Tecniplast |
|---|---|
| Current animal core facility/
or for laboratory purposes: | Zebrafish breeding for purposes of the Department |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | Department staff (including 1 animal caretaker), students |
| Services: | Fish breeding and keeping, ensuring animal welfare, monitoring animal
health |
| Research conducted using the zebrafish model, grants: | Comparative analysis of myogenesis during normal development and in pathological states.
Grants implemented based on Zebrafish Research Facility animals: • A new animal model of human McArdle’s disease in zebrafish (Danio rerio). Project PI: Marta Migocka-Patrzałek, Project number: 2021/43/D/NZ4/00081. Funding institution: National Science Centre, Sonata 17 • In vitro assessment of the effect of selected cytostatic substances of natural origin on the structure and cell cycle of embryonal rhabdomyosarcoma. Project PI: Małgorzata Daczewska. Funding: IDUB program, University of Wrocław • The influence of substances with therapeutic potential on the animal model of human McArdle’s disease. Project PI: Marta Migocka-Patrzałek, Funding: IDUB program, University of Wrocław • Functional analysis of HSPB8 in model organisms (Danio rerio and Drosophila melanogaster) with particular emphasis on pathological states associated with human orthologue disorders. Funding: NCN, Sonata 7, Project PI: Magda Dubińska-Magiera • The role of NWC in the structure and function of cell cilia in zebrafish. Project PI: Joanna Niedbalska-Tarnowska, Funding: MINIATURA, NCN • Evaluation of the impact of transient silencing of muscle glycogen phosphorylase (Pygm) expression on the structure of zebrafish muscles. Funding: NCN, Miniatura Project, PI: Marta Migocka-Patrzałek • e-zebrafish. Dive into knowledge! Funding: FNP, eNgage, Project leader: Magda Dubińska-Magiera |
| Specialized equipment: | |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains:
● AB |
| Zebrafish housing system capacity (e.g. number of racks): | 8 racks: 400 small (3.5 l) or 200 large (8 l) tanks |
|---|---|
| Current animal core facility/
or for laboratory purposes: | The Zebrafish Core Facility provides Jagiellonian University (UJ) staff and non-UJ members with access to larval and adult zebrafish to conduct research using this laboratory model organism. |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | 3 staff members, 6 postdocs, 7 PhD students, 2 technicians |
| Services: | Providing zebrafish larvae and adult fish for experiments.
Keeping different zebrafish lines and mutants. |
| Research conducted using the zebrafish model, grants: | Studies of the mechanisms of immune response following infection of zebrafish with bacterial and viral pathogens.
Zebrafish as a model for human infectious and cardiovascular diseases. Studies of the immune-neuroendocrine interactions. |
| Specialized equipment: | Confocal microscope for zebrafish real-time imaging, high quality fluorescence stereomicroscopes, optical, fluorescence and inverted microscopes, computer image analysis system.
Four pneumatic microinjectors and micromanipulators required for zebrafish injections. Spectral flow cytometer with cell-sorting system (FACS). Rotor-Gene qRT-PCR machines, PCR thermocyclers, laminar hoods, CO2-incubators including cooled incubator for fish cell culture, ultracentrifuges, centrifuges, microplate reader for absorbance and fluorescence measurements, apparatus for electrophoresis. System to study zebrafish behaviour (Noldus). |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains:
● Tuebingen ● AB ● AB-TL ● CASPER Transgenics: ● Tg(mpx:GFP) ● Tg(mpeg1:mCherry) ● Tg(kdrl:mTurquoise) ● Tg(CMV:GFP-Lc3) ● Tg(fli1:EGFP) ● Tg(NeuroD:EGFP) ● Tg(Olig2:GFP) Mutants/Crispants: ● myd88 |
| Zebrafish housing system capacity (e.g. number of racks): | Glass tank |
|---|---|
| Current animal core facility/
or for laboratory purposes: | Fish facility of Mossakowski Medical Research Institute, Polish Academy of Sciences |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | 1 |
| Services: | • Fish maintenance, spawning (with egg collection)
• Acute toxicity test according to OECD 246, • Imaging and morphometric analysis, • Microinjections (if cancer cells, also cell maintenance in culture, banking and possible in vitro testing, if bacteria, we can culture/bank class I and II bacteria), • Assistance with CRISPR/Cas9 strategy design and gRNA synthesis, • Phenotyping and genotyping, • Setting up methods according to user needs, • Assistance with fish import/export, • Reporting to the Ministry of Science and Higher Education and KKE, preparation and delivery of training courses, • Practical training for users working with zebrafish, • Expert advice on setting up fish facilities and using Danio for scientific or educational purposes. |
| Research conducted using the zebrafish model, grants: | • toxicity studies
• research on rare diseases • teaching and demonstration classes • services for external bodies ('undisclosed' details) |
| Specialized equipment: | • Microinjector FemtoJet-4i
• Stereoscope Zeiss Stemi 508 • Camera Zeiss Axiocam 105 colour • Zeiss AxioCam ICm1 camera • NIGHTSEA (EMS) with SFA-LFS-GR, SFA-RB-GO and Dual Switch Box Kit • Incubators INCU-LINE IL-56 (in BSL-I and BSL-II laboratories) • Egg incubator and hatcher (Mamrotte) • Levenhuk DTX RC2 digital microscope (for laminar hood work) |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains:
• AB Transgenics: • Currently no lines Mutants/Crispants: • Currently no lines |
| Zebrafish housing system capacity (e.g. number of racks): | |
|---|---|
| Current animal core facility/
or for laboratory purposes: | |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | |
| Services: | |
| Research conducted using the zebrafish model, grants: | |
| Specialized equipment: | |
| Available zebrafish strains, transgenic lines, mutants: |
| Zebrafish housing system capacity (e.g. number of racks): | 2 racks (1regular rack + quarantine rack) |
|---|---|
| Current animal core facility/
or for laboratory purposes: | Facility for the needs of the University and others |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | 2 technicians |
| Services: | Breeding and maintaining fish |
| Research conducted using the zebrafish model, grants: | Toxicity screening |
| Specialized equipment: | • DanioVision with EthoVision XT software (Noldus) • Microinjector (Eppendorf FemtoJet-4i) • Incubator |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains:
● AB |
| Zebrafish housing system capacity (e.g. number of racks): | none |
|---|---|
| Current animal core facility/
or for laboratory purposes: | Electron Paramagnetic Resonance laboratory |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | 2 |
| Services: | In vivo measurements of membrane fluidity in zebrafish embryos, in vivo melanin radical screening in normal and melanoma -injected zebrafish embryos, oxidative stress (free radicals identification, antioxidant properties) and membrane fluidity in zebrafish cells and tissues. |
| Research conducted using the zebrafish model, grants: | Grant – Developing specially-shaped capillary for in vivo X-band EPR measurements of zebrafish embryos
Projects: ● pro-oxidant and antioxidant activity of agri-food waste using in vivo zebrafish embryo model ● melanin and melanoma screening in in vivo zebrafish embryos ● influence of oxidative stress and DMSO on membrane fluidity in vivo in zebrafish embryos |
| Specialized equipment: | High sensitivity Electron Paramangetic Resonance spectrometer |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains:
● none Transgenics: ● none Mutants/Crispants: ● none |
| Zebrafish housing system capacity (e.g. number of racks): | No racks, fish kept in a glass aquarium |
|---|---|
| Current animal core facility/
or for laboratory purposes: | For laboratory purposes |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | Two persons who provide animal care, the designated veterinarian |
| Services: | Breeding and maintenance of fish, animal welfare, reporting to the Ministry of Science and Higher Education, shifts at weekends and holiday, taking care of the equipment in the breeding/laboratory, replenishment consumables on an ongoing basis,working with animals in accordance with AAALAC recommendations |
| Research conducted using the zebrafish model, grants: | Toxicity tests according to the OECD guidelines as well as in accordance with the principles of Good Laboratory Practice (GLP), research projects, services for external entities |
| Specialized equipment: | Stereoscopic microscope, laboratory incubator |
| Available zebrafish strains, transgenic lines, mutants: | Wild type strains: AB_OMD
● Transgenics: --- ● Mutants/Crispants:---- |
| Zebrafish housing system capacity (e.g. number of racks): | |
|---|---|
| Current animal core facility/
or for laboratory purposes: | For experiments only;
the laboratory has applied to the Ministry of the Environment to add it to the list of Users of Danio rerio (and the inspections have been completed) |
| Staff / zebrafish caretakers (number of people, incl. technicians/students): | |
| Services: | |
| Research conducted using the zebrafish model, grants: | Pharmacological and toxicological studies are planned |
| Specialized equipment: | ViewPoint system, MicroZebraLab system, CardioFish system, access to the Leica Stellaris 8 confocal microscope and LightSheet microscope in cooperation with CDT-CARD |
| Available zebrafish strains, transgenic lines, mutants: |
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