6th workshop of the Polish Zebrafish Society  
Institute of Zoology and Biomedical Research of Jagiellonian University in Krakow
14-16 February 2024

Program
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Thank you for your feedback in evaluation survey!!!

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we will use your suggestions during organization next events.

Here are some of your comments which make us especially happy:

“Excellent course! I learned a lot in wonderful atmosphere :)”

PZS is the friendliest society that I have the pleasure of being a member of. I'm very impressed. The workshops exceeded my expectations. Thank you very much for the opportunity to participate, because it will certainly help us in our research. And I hope to share the results at our future meetings :-)”

“Great workshops, great people, and atmosphere. Keep that going!”

„A very good idea to integrate society of people working with zebrafish. It would be good to somehow continuate integration by regular meetings/conferences.”

“It was fun experience; I hope I will come back next year :)”

„You're the best :)”


Polish Zebrafish Society

The workshops will be held at the Institute of Zoology and Biomedical Research of the Jagiellonian University in Kraków (ul. Gronostajowa 9, 30-387 Kraków, Poland).
The training is intended for all scientists interested in research using zebrafish (Danio rerio) as a model organism.

Mobirise

During the training, there will be open lectures, which do not require registration, on research on human diseases using zebrafish as a model organism (1:00 - 5:00 p.m. on the first day of the Workshop, February 14, 2024).

Over the next two days, there will be practical exercises aimed at familiarizing participants with this model organism, its husbandry, possibilities of use in research, basic research techniques used in science, including toxicological tests, microinjections – also injections of cancer cells (zebrafish xenograft model), microscopic imaging using Light Sheet technology, behavioral analyses, use of gene editing technologies such as CRISPR-Cas9 method, genotyping and more.
People interested in participating in the training are asked to send an application and a short cover letter (up to 250 words) to zebrafish.pl@gmail.com by January 15, 2024. Qualified applicants will be asked to prepare a poster on their current research projects/interests. In the case of a large number of applicants, exceeding the capacity to properly conduct the training, participants will be selected based on the above-mentioned cover letter.


Date: 14-16/02/2024
Registration for workshops: until January 15, 2024
Date of announcement of the list of qualified participants: by January 25, 2024
Fee: 150 PLN for PTZ members who have paid the membership fee for 2024; 200 PLN for non-PTZ members. The fee covers participation in training, coffee breaks, and lunches). If you are joining the Society, it is possible to allocate part of the fee towards the membership fee for 2024.
Organizers: Management Committee of the Polish Zebrafish Society



Poster formatting
- All posters must be prepared in English.
- All posters must be displayed in PORTRAIT (vertical) format.
- Poster boards will accommodate A0 size posters: height 1189 mm (46.8 inches), width 841 mm (33.1 inches). Please ensure that your display will fit within these parameters. You may choose to go for the smaller size, having regard to the poster legibility.
- Do not include bibliography.
- Use sans-serif font, preferentially Verdana.

Program

preliminary


DAY I  (open to public)

13.00-17.30 LECTURES
13:00 -13:10 - "Welcome and Introduction" - Przemko Tylżanowski - Chairmen of Polish Zebrafish Society, Medical University of Lublin, KU Leuven, Belgium
13:10 - 13:40 - “The zebrafish as a model for scientific research” - Marta Migocka-Patrzałek -University of Wroclaw
13:40 - 14:10- "Zebrafish xenografts in cancer research - High-content screening to identify drug combinations effective against Ewing sarcoma"- Martin Distel- Medical University of Vienna
14:10 - 14:40 -  "Zebrafish model to study brain development and diseases" – Justyna Zmorzyńska - The International Institute of Molecular Mechanisms and Machines
14:40 - 15:10 - “Zebrafish as a Model Organism for Toxicity Studies: Exploiting Advantages in Biomedical Research” - Barbara Budzyńska - Medical University of Lublin
~ 20 min break ~ 
15:30 - 16:00 -"Zebrafish in the lens of behavioral research"- Małgorzata Potoczna – Transpharmation Poland, University of Warmia and Mazury in Olsztyn
16:00 - 16:30 -  "Zebrafish larvae infection model to study host-pathogen interactions" - Magdalena Widziołek-Pooranachandran - Jagiellonian University
16:30 - 17:00 - "Modeling of viral infections in fish:from fundamental to applied science" - Krzysztof Rakus-Jagiellonian University
17:00 - 17:30 "Evolution of genome editing in zebrafish: from origins to advances and integration into translational medicine" - Anna Sarosiak -Institute of Physiology and Pathology of Hearing
17.30 - 20.00 Poster session/ get together 

DAY II 
8.00-8.15 Registration and Welcome
8.15-10.15 Practicals - part I
10:15-10:30 Coffee break
10.30-12.30 Practicals -part II
12.30-14.00 Lunch & Discussion
14:00-16:00 Practicals -part III
16:00-16:15 Coffee break
16.15-18.15 Practicals -part IV


DAY III 
9.00- 10.00 Practicals - part V
10.00-11.00 Practicals - part VI
11:00-11:15 Coffee break
11.15-12.15 Practicals - part VII
12.15-13.15 Practicals - part VIII
13.15-14.45 Lunch & Summary

Practical parts schedule

DAY II

TimeGroup IGroup IIGroup IIIGroup IV
08.15– 10.15Toxicity screening using zebrafish embryos (LiCl) - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Joanna Dybalska, Mikołaj Mazur)
Basics of imaging and sample preparation - BF/fluorescence (Piotr Podlasz, Anna Sarosiak)
Microinjections (basics)
(Przemko Tylzanowski, Tomasz Prajsnar)
+ Zebrafish xenograft model (Anna Boguszewska-Czubara)
Behaviour (Agnieszka Michalak, Małgorzata Potoczna)
+ Visit in the zebrafish facility (Dariusz Gajdziński)
10.15- 10.30Short breakShort breakShort break

Short break

10.30-12.30Behaviour (Agnieszka Michalak, Małgorzata Potoczna)
+ Visit in the zebrafish facility (Dariusz Gajdziński)

Toxicity screening using zebrafish embryos (LiCl) - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Joanna Dybalska, Mikołaj Mazur)
Basics of imaging and sample preparation - BF/fluorescence (Piotr Podlasz, Anna Sarosiak)Microinjections (basics)
(Przemko Tylzanowski, Tomasz Prajsnar)
+ Zebrafish xenograft model (Anna Boguszewska-Czubara)


12.30-14.00Lunch & Discussion


Lunch & Discussion

Lunch & Discussion Lunch & Discussion
14.00-16.00Microinjections (basics)
(Przemko Tylzanowski, Tomasz Prajsnar)
+ Zebrafish xenograft model (Anna Boguszewska-Czubara)
Behaviour (Agnieszka Michalak, Małgorzata Potoczna)
+ Visit in the zebrafish facility (Dariusz Gajdziński)
Toxicity screening using zebrafish embryos (LiCl) - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Joanna Dybalska, Mikołaj Mazur)
Basics of imaging and sample preparation - BF/fluorescence (Piotr Podlasz, Anna Sarosiak)
16.00-16.15Short break

Short break

Short break

Short break
16.15-18.15Basics of imaging and sample preparation - BF/fluorescence (Piotr Podlasz, Anna Sarosiak) Microinjections (basics)
(Przemko Tylzanowski, Tomasz Prajsnar)
+ Zebrafish xenograft model (Anna Boguszewska-Czubara)

Behaviour (Agnieszka Michalak, Małgorzata Potoczna)
+ Visit in the zebrafish facility (Dariusz Gajdziński)

Toxicity screening using zebrafish embryos (LiCl) - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Joanna Dybalska, Mikołaj Mazur)

DAY III

TimeGroup IGroup IIGroup IIIGroup IV
9:00 - 10:00Toxicity screening using zebrafish embryos (LiCl) - Part II (Natalia Kasica)

Introduction to cell sorting (Katarzyna Kłak-Pomykała)Visualisation of xenografts and transgenic zebrafish (confocal microscopy)
(Piotr Podlasz)
CRISPR/Cas9 design (Anna Sarosiak)
personal laptop required!
10:00 - 11:00CRISPR/Cas9 design (Anna Sarosiak)
personal laptop required!
Toxicity screening using zebrafish embryos (LiCl) - Part II (Natalia Kasica)Introduction to cell sorting (Katarzyna Kłak-Pomykała)

Visualisation of xenografts and transgenic zebrafish (confocal microscopy)
(Piotr Podlasz)
11:00 - 11:15Short break

Short break Short break

Short break

11:15 - 12:15Visualisation of xenografts and transgenic zebrafish (confocal microscopy)
(Piotr Podlasz)
CRISPR/Cas9 design (Anna Sarosiak)
personal laptop required!

Toxicity screening using zebrafish embryos (LiCl) - Part II (Natalia Kasica) Introduction to cell sorting (Katarzyna Kłak-Pomykała)

12:15 - 13:15Introduction to cell sorting (Katarzyna Kłak-Pomykała)

Visualisation of xenografts and transgenic zebrafish (confocal microscopy)
(Piotr Podlasz)
CRISPR/Cas9 design (Anna Sarosiak)
personal laptop required!
Toxicity screening using zebrafish embryos (LiCl) - Part II (Natalia Kasica)

13:15 - 14:45Lunch & Summary

Lunch & Summary

Lunch & SummaryLunch & Summary

Lecturers
and practical parts tutors

Przemko Tylzanowski 
Medical University of Lublin
KU Leuven, Belgium
przemko@kuleuven.be

Przemko Tylzanowski

Marta Migocka-Patrzałek
University of Wroclaw
marta.migocka-patrzalek@uwr.edu.pl 

Marta Migocka-Patrzalek

Martin Distel
Medical University of Vienna; St.
Anna Children’s Cancer Research
Institute (CCRI), Vienna
martin.distel@ccri.at

Martin Distel

Justyna Zmorzyńska 
The International Institute
of Molecular Mechanisms and
Machines
j.zmorzynska@imol.institute

Justyna Zmorzynska

Barbara Budzyńska
Medical University of Lublin
barbara.budzynska@umlub.pl 

Barbara Budzynska

Małgorzata Potoczna 
Transpharmation Poland,
University of Warmia and Mazury in Olsztyn 
malgorzata.potoczna@transpharmation.co.uk

Malgorzata Potoczna

Magdalena Widziołek- Pooranachandran
Jagiellonian University,
Krakow
magdalena.widziolek@uj.edu.pl

Magdalena Widziołek- Pooranachandran

Krzysztof Rakus
Jagiellonian University,
Krakow
krzysztof.rakus@uj.edu.pl 

Krzysztof Rakus

Anna Sarosiak 
Institute of Physiology and Pathology of Hearing
anna.sarosiak@gmail.com 

Anna Sarosiak

Anna Boguszewska-Czubara 
Medical University of Lublin
anna.boguszewska-czubara@am.lublin.pl  

Anna Boguszewska-Czubara

Piotr Podlasz
University of Warmia and Mazury in Olsztyn 
piotr.podlasz@uwm.edu.pl 

Piotr Podlasz

Natalia Kasica 
University of Warmia and Mazury in Olsztyn 
natalia.kasica@uwm.edu.pl

Natalia Kasica

Joanna Dybalska
University of Warmia and Mazury in Olsztyn 
joanna.dybalska@uwm.edu.pl

Joanna Dybalska

Agnieszka Michalak
Medical University of Lublin
agnieszka.michalak@umlub.pl

Agnieszka Michalak

Dariusz Gajdziński
Jagiellonian University,
Krakow
dariusz.gajdzinski@uj.edu.pl

Dariusz Gajdziński

Tomasz Prajsnar 
Jagiellonian University,
Krakow
tomasz.prajsnar@uj.edu.pl

Tomasz Prajsnar

Katarzyna
Kłak-Pomykała
Jagiellonian University,
Krakow
katarzyna.klak@doctoral.uj.edu.pl

Katarzyna Kłak-Pomykała

Mikołaj Mazur
Jagiellonian University,
Krakow
mikolaj.mazur@doctoral.uj.edu.pl;

Mobirise

Sponsors

We thank our generous sponsor

Address

Kazimierza Jaczewskiego 8 str                     
Lublin, 20-090

Contacts

Email: zebrafish.pl@gmail.com

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