We are pleased to invite you to the 7th Workshop of the Polish Zebrafish Society, which will take place in Olsztyn from 25 to 27 June 2025. The workshop will be held at the Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, (ul. Oczapowskiego 13 and 14).
The training is intended for all scientists interested in research using zebrafish (Danio rerio) as a model organism. It will provide participants with both theoretical knowledge and practical skills essential for working with zebrafish in various scientific fields, including genetics, developmental biology, toxicology, and biomedical research.
Program
The workshop will consist of open lectures and practical sessions:
Open Lectures (Day 1, 1:00 - 5:00 p.m.): No registration required. These lectures will focus on using zebrafish as a model organism for studying human diseases. A poster session will follow until 8:00 p.m..
Practical Training (Days 2-3): Hands-on sessions covering essential zebrafish research techniques, including:
Husbandry and breeding
Toxicological testing
Microinjections (including zebrafish xenograft model)
Advanced microscopy
Behavioral analyses
CRISPR-Cas9 gene editing
Genotyping and more
Registration
Participants interested in attending the practical sessions should send an application and a short cover letter (max. 250 words) to zebrafish.pl@gmail.com by May 18, 2025. Due to limited capacity, applicants will be selected based on their cover letter.
Registration deadline: May 18, 2025
Announcement of selected participants: May 25, 2025
Fee: 150 PLN for PTZ members (who have paid the 2025 membership fee), 200 PLN for non-members. The fee covers training participation, coffee breaks, and lunches. New members may allocate part of the fee toward the PTZ membership.
Poster Session
Selected participants will be required to prepare a poster on their research projects. Posters must:
be in English
be in portrait format (A0 size: 1189 mm height x 841 mm width)
exclude bibliography
use a sans-serif font (preferably Verdana)
Organizers
The workshop is organized by the Management Committee of the Polish Zebrafish Society.
We look forward to seeing you in Olsztyn!
DAY I 25.06.25
9:00 - 12:00 "Design Thinking" Workshop (registered participants)
13.00-17.30 LECTURES open to public
(including coffee break)
17.30 - 20.00 Poster session/ get together
DAY II 26.06.25
8.00-8.15 Registration and Welcome
8.15-10.15 Practicals - part I
10:15-10:30 Coffee break
10.30-12.30 Practicals -part II
12.30-14.00 Lunch & Discussion
14:00-16:00 Practicals -part III
16:00-16:15 Coffee break
16.15-18.15 Practicals -part IV
19.00- Social event
DAY III 27.06.25
9.00- 10.15 Practicals - part V
10.15-11.30 Practicals - part VI
11:30-11:45 Coffee break
11.45-13.00 Practicals - part VII
13:00-14.15 Practicals - part VIII
14.15-15.30 Lunch & Summary
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 08.15– 10.15 | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) | Imaging and sample preparation - Fluorescence, confocal, lightfield microscopy, LSM Lightfield 4D - Zeiss, VAST FluoroImager, Union Biometrica (Piotr Podlasz) | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) |
| 10.15- 10.30 | Coffee break | Coffee break | Coffee break | Coffee break |
| 10.30-12.30 | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) | Basics of imaging and sample preparation - BF/fluorescence (Piotr Podlasz, Anna Sarosiak) | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) |
| 12.30-14.00 | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion |
| 14.00-16.00 | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) | Basics of imaging and sample preparation - BF/fluorescence (Piotr Podlasz, Anna Sarosiak) |
| 16.00-16.15 | Short break | Short break | Short break | Short break |
| 16.15-18.15 | Basics of imaging and sample preparation - BF/fluorescence (Piotr Podlasz, Anna Sarosiak) | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) |
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 9:00 - 10:15 | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) | Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity (Piotr Podlasz) | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! |
| 10:15 - 11:30 | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) | Visualisation of xenografts and Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity
(Piotr Podlasz) |
| 11:30 - 11:45 | Coffee break | Coffee break | Coffee break | Coffee break |
| 11:45 - 13:00 | Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity
(Piotr Podlasz) | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) |
| 13:00 - 14:15 | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) | Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity
(Piotr Podlasz) | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) |
| 14:15 - 15:30 | Lunch & Summary | Lunch & Summary | Lunch & Summary | Lunch & Summary |
Przemko Tylzanowski
Medical University of Lublin
KU Leuven, Belgium
przemko@kuleuven.be
Darius Balciunas
Temple University College of Science and Technology
darius@temple.edu
Justyna Zmorzyńska
The International Institute
of Molecular Mechanisms and
Machines
j.zmorzynska@imol.institute
Tomasz Prajsnar
Jagiellonian University,
Krakow
tomasz.prajsnar@uj.edu.pl
Katerina Makarova
Medical University of Warsaw
katerina.makarova@wum.edu.pl
Piotr Podlasz
University of Warmia and Mazury in Olsztyn
piotr.podlasz@uwm.edu.pl
Małgorzata Potoczna
Transpharmation Poland,
University of Warmia and Mazury in Olsztyn
malgorzata.potoczna@transpharmation.co.uk
Małgorzata Chmielewska-Krzesińska
University of Warmia and Mazury in Olsztyn
malgorzata.chmielewska@uwm.edu.pl
Natalia Kasica
University of Warmia and Mazury in Olsztyn
natalia.kasica@uwm.edu.pl
Joanna Dybalska
University of Warmia and Mazury in Olsztyn
joanna.dybalska@uwm.edu.pl
Anna Sarosiak
University Center for Women's and Newborn Health
Medical University of Warsaw, Poland
anna.sarosiak@gmail.com
Mikołaj Mazur
Jagiellonian University,
Krakow
mikolaj.mazur@doctoral.uj.edu.pl;
We thank our generous sponsor
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