We are pleased to invite you to the 7th Workshop of the Polish Zebrafish Society, which will take place in Olsztyn from 25 to 27 June 2025. The workshop will be held at the Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, (ul. Oczapowskiego 13 and 14).
The training is intended for all scientists interested in research using zebrafish (Danio rerio) as a model organism. It will provide participants with both theoretical knowledge and practical skills essential for working with zebrafish in various scientific fields, including genetics, developmental biology, toxicology, and biomedical research.
Program
An independent workshop "Design Thinking in Research" will be held before the 7th Workshop of the Polish Zebrafish Society (Day 1, 9:00 - 12:00 a.m) .
The workshop will consist of open lectures and practical sessions:
Open Lectures (Day 1, 1:00 - 5:30 p.m.): No registration required. These lectures will focus on using zebrafish as a model organism for studying human diseases. A poster session will follow until 8:00 p.m..
Practical Training (Days 2-3): Hands-on sessions covering essential zebrafish research techniques, including:
Husbandry and breeding
Toxicological testing
Microinjections (including zebrafish xenograft model)
Advanced microscopy
Behavioral analyses
CRISPR-Cas9 gene editing
Genotyping and more
Registration
Participants interested in attending the practical sessions should send an application and a short cover letter (max. 250 words) to zebrafish.pl@gmail.com by May 22, 2025. Due to limited capacity, applicants will be selected based on their cover letter.
Registration deadline: May 22, 2025
Announcement of selected participants: May 25, 2025
Fee: 150 PLN for PTZ members (who have paid the 2025 membership fee), 200 PLN for non-members. The fee covers training participation, coffee breaks, and lunches. New members may allocate part of the fee toward the PTZ membership.
Poster Session
Selected participants will be required to prepare a poster on their research projects. Posters must:
be in English
be in portrait format (A0 size: 1189 mm height x 841 mm width)
exclude bibliography
use a sans-serif font (preferably Verdana)
Organizers
The workshop is organized by the Management Committee of the Polish Zebrafish Society.
We look forward to seeing you in Olsztyn!
🧠 Research meets creativity – join the Design Thinking in Research workshop!
The Polish Zebrafish Society warmly invites all interested researchers to participate in a free, hands-on workshop focused on applying Design Thinking in academic research.
📅 Date: June 25, 2025
🕘 Time: 9:00–12:00
📍 Venue: Prof. M. Tarczyński Auditorium, Oczapowskiego 13/1, Olsztyn
🔢 Capacity: 40 participants (first come, first served)
💰 Participation is free of charge!
What will you gain?
Participants will explore how to:
identify real user needs in the research process,
generate and test research ideas creatively,
collaborate effectively in interdisciplinary teams,
enhance the practical and social impact of scientific work.
The workshop is fully hands-on – guiding participants through all Design Thinking stages: empathy, problem definition, ideation, prototyping, and testing.
📎 No prior experience required – just bring curiosity and a notebook!
DAY I 25.06.25
9:00 - 12:00 "Design Thinking" Workshop
13.00-17.30 LECTURES open to public
(including coffee break)
13:00 -13:10 - Welcome and Introduction - Przemko Tylżanowski - Chairman of Polish Zebrafish Society,
13:10 -13:40 - “Big fish in a big pond or what can we fish out using zebrafish” - Przemko Tylżanowski -Medical University of Lublin, KU Leuven, Belgium
13:40 -14:10 - "Zebrafish on Alert: Tracking Toxicity from Molecule to Behavior" - Piotr Podlasz - University of Warmia and Mazury in Olsztyn
14:10 -14:40 - "How can we model human neuropsychiatric disorders using zebrafish?" - Justyna Zmorzyńska - The International Institute of Molecular Mechanisms and Machines, Warsaw
14:40 -15:10 - "Zebrafish in the lens of behavioral research"- Małgorzata Potoczna - Transpharmation Poland, University of Warmia and Mazury in Olsztyn
~ 20 min break
15:30 -16:00 - "Homology directed repair using oligonucleotide templates: from epitope tagging to conditional mutagenesis"- Darius Balciunas - Life Sciences Center, Vilnius University, Lithuania; Temple University College of Science and Technology, Philadelphia, USA
16:00 -16:30 - "Watching Immunity -using zebrafish and microscopy to understand the immune response" - Tomasz Prajsnar - Jagiellonian University, Krakow
16:30 -17:00" - "Zebrafish Xenograft Models: Small Fish, Big Impact in Cancer Research" - Małgorzata Chmielewska-Krzesińska - University of Warmia and Mazury in Olsztyn
17:00 -17:30 - “Beneath the stripes: zebrafish welfare and breeding explained” - Magdalena Gral - Zebrafish Core Facility, International Institute of Molecular and Cell Biology in Warsaw
17.30 - 20.00 Poster session/ get together
DAY II 26.06.25
8.00-8.15 Registration and Welcome
8.15-10.15 Practicals - part I
10:15-10:30 Coffee break
10.30-12.30 Practicals -part II
12.30-14.00 Lunch & Discussion
14:00-16:00 Practicals -part III
16:00-16:15 Coffee break
16.15-18.15 Practicals -part IV
19.00- Social event
DAY III 27.06.25
9.00- 10.15 Practicals - part V
10.15-11.30 Practicals - part VI
11:30-11:45 Coffee break
11.45-13.00 Practicals - part VII
13:00-14.15 Practicals - part VIII
14.15-15.30 Lunch & Summary
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 08.15– 10.15 | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) | Imaging and sample preparation - Fluorescence, confocal, lightfield microscopy, LSM Lightfield 4D - Zeiss, VAST FluoroImager, Union Biometrica (Piotr Podlasz) | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) |
| 10.15- 10.30 | Coffee break | Coffee break | Coffee break | Coffee break |
| 10.30-12.30 | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) | Imaging and sample preparation - Fluorescence, confocal, lightfield microscopy, LSM Lightfield 4D - Zeiss, VAST FluoroImager, Union Biometrica (Piotr Podlasz) | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) |
| 12.30-14.00 | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion |
| 14.00-16.00 | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) | Imaging and sample preparation - Fluorescence, confocal, lightfield microscopy, LSM Lightfield 4D - Zeiss, VAST FluoroImager, Union Biometrica (Piotr Podlasz) |
| 16.00-16.15 | Short break | Short break | Short break | Short break |
| 16.15-18.15 | Imaging and sample preparation - Fluorescence, confocal, lightfield microscopy, LSM Lightfield 4D - Zeiss, VAST FluoroImager, Union Biometrica (Piotr Podlasz) | Microinjections and Zebrafish xenograft model, Injectors WPI, RWD (Małgorzata-Chmielewska Krzesińska) | Behavioral analysis, Noldus, ViewPoint (Małgorzata Poczna)
+ Visit in the zebrafish facility (Joanna Dybalska-Szczepanek) | Toxicity screening using zebrafish embryos - Part I (Natalia Kasica)
+ Dissection of organs from adult zebrafish, finclips and swabbing (Mikołaj Mazur) |
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 9:00 - 10:15 | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) | Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity (Piotr Podlasz) | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! |
| 10:15 - 11:30 | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) | Visualisation of xenografts and Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity
(Piotr Podlasz) |
| 11:30 - 11:45 | Coffee break | Coffee break | Coffee break | Coffee break |
| 11:45 - 13:00 | Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity
(Piotr Podlasz) | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) |
| 13:00 - 14:15 | Microinjections II, Automated microinjection system for zebrafish, Life Science Methods (Małgorzata-Chmielewska Krzesińska) | Visualisation of xenografts and transgenic zebrafish, Automated, high throughput microscopy, ACQUIFER, Revvity
(Piotr Podlasz) | CRISPR/Cas9 design (Anna Sarosiak) personal laptop required! | Toxicity screening using zebrafish embryos - Part II (Natalia Kasica) |
| 14:15 - 15:30 | Lunch & Summary | Lunch & Summary | Lunch & Summary | Lunch & Summary |
Przemko Tylzanowski
Medical University of Lublin
KU Leuven, Belgium
przemko@kuleuven.be
Darius Balciunas
Life Sciences Center, Vilnius University, Lithuania
Temple University College of Science and Technology, Philadelphia, USA
darius@temple.edu
Justyna Zmorzyńska
The International Institute
of Molecular Mechanisms and
Machines, Warsaw
j.zmorzynska@imol.institute
Tomasz Prajsnar
Jagiellonian University,
Krakow
tomasz.prajsnar@uj.edu.pl
Magdalena Gral
Zebrafish Core Facility
International Institute of Molecular and Cell Biology in Warsaw
mgral@iimcb.gov.pl
Katerina Makarova
Medical University of Warsaw
katerina.makarova@wum.edu.pl
Piotr Podlasz
University of Warmia and Mazury in Olsztyn
piotr.podlasz@uwm.edu.pl
Małgorzata Potoczna
Transpharmation Poland,
University of Warmia and Mazury in Olsztyn
malgorzata.potoczna@transpharmation.co.uk
Małgorzata Chmielewska-Krzesińska
University of Warmia and Mazury in Olsztyn
malgorzata.chmielewska@uwm.edu.pl
Natalia Kasica
University of Warmia and Mazury in Olsztyn
natalia.kasica@uwm.edu.pl
Joanna Dybalska
University of Warmia and Mazury in Olsztyn
joanna.dybalska@uwm.edu.pl
Anna Sarosiak
University Center for Women's and Newborn Health
Medical University of Warsaw, Poland
anna.sarosiak@gmail.com
Mikołaj Mazur
Jagiellonian University,
Krakow
mikolaj.mazur@doctoral.uj.edu.pl;
We thank our generous sponsor
AI Website Maker