Przemko Tylzanowski
Medical University of Lublin, Poland
University of Leuven, Belgium
przemko@kuleuven.be
DAY I (30.11.2017)
8.30-9.00 Registration
9.00-9.30 Welcome and Introduction to Practical Part
9.30-13.30 Practicals - part I– coordinator: Piotr Podlasz (University of Warmia and Mazury in Olsztyn)
13.30-14.30 Lunch
LECTURES (open to public)
15.00-15.30 Introduction to zebrafish – Przemko Tylżanowski (Medical University of Lublin /University of Leuven)
15.30-16.00 Legal aspects (Lecture in Polish)- Mirosław Łańcut (Medical University of Lublin)
16.00-16.30 The Zebrafish model: 3R in husbandry and laboratory practice (International Institute of Molecular and Cell Biology in Warsaw)
16.30-16.45 Coffee break
16.45- 17.15 Alternative model - zebrafish in toxicology and neurotoxicology - Róbert Kovács (Animalab, Hungary)
17.15-17.35 Complete solution for high-throughput zebrafish larvae tracking - Patrick H. Zimmerman, (Noldus Information Technology, Wageningen, The Netherlands).
17.35-17.55 Aquaneering - zebrafish housing - Róbert Kovács (Animalab, Hungary)
18.00 – 20.00 Poster session/ Wine & cheese party
DAY II (01.12.2017)
9.00-12.30 Practicals - part II 12.30-13.30 Lunch & Discussion
13.30-14.50 Practicals - part III
14.50-15.00 Closing remarks and departure
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 09.30– 10.30 | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) | Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) | Automated observation and tracking of larval zebrafish
(Patrick Zimmerman) | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) |
| 10.30- 11.30 | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) | Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) | Automated observation and tracking of larval zebrafish
(Patrick Zimmerman) |
| 11.30-12.30 | Automated observation and tracking of larval zebrafish
(Patrick Zimmerman) | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) | Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) |
| 12.30-13.30 | Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) | Automated observation and tracking of larval zebrafish
(Patrick Zimmerman) | Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk) | Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica) |
| Time | Group I | Group II | Group III | Group IV |
|---|---|---|---|---|
| 9.00-9.50 | Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica) | Dissection of organs from adult zebrafish (Anna Jakimiuk) | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) | Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) |
| 9.50-10.40 | Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) | Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica) | Dissection of organs from adult zebrafish (Anna Jakimiuk) | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) |
| 10.40-11.30 | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) | Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) | Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica) | Dissection of organs from adult zebrafish (Anna Jakimiuk) |
| 11.30-12.20 | Dissection of organs from adult zebrafish (Anna Jakimiuk) | Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska) | Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger) | Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica) |
| 12.30-13.30 | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion | Lunch & Discussion |
| 13.30-15.50 | Time for repetition of - selected issues | Time for repetition of - selected issues | Time for repetition of - selected issues | Time for repetition of - selected issues |
Przemko Tylzanowski
Medical University of Lublin, Poland
University of Leuven, Belgium
przemko@kuleuven.be
Mirosław Łańcut
Medical University of Lublin, Poland
miroslawlancut@umlub.pl
Małgorzata Wiweger
International Institute of Molecular and Cell Biology
in Warsaw, Poland
mwiweger@iimcb.gov.pl
Róbert Kovács
Animalab, Hungary
Patrick Zimmerman
Noldus Information Technology, Wageningen,
The Netherlands
Practical parts coordinator
Piotr Podlasz
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland
piotr.podlasz@uwm.edu.pl
Anna Jakimiuk
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland
annajakimiuk@wp.pl
Natalia Kasica-Jarosz
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn
natalia_kasica@wp.pl
Aneta Chełstowska-Wejman
Eppendorf
Agnieszka Jakubaszek
Medical University of Lublin, Poland
Anna Sobolewska
Medical University of Lublin, Poland
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