We are pleased to invite you to the 1st CRISPR/Cas9 Practical Course, organised by the Polish Zebrafish Society. This three-day programme will provide participants with the theoretical knowledge and basic practical skills essential for working with CRISPR/Cas9 gene editing method. The practical part of the course will take place in Krakow on 16 and 17 July 2026. The course is available only for the PTZ members .
Schedule and the venue
The theoretical part of the course will be held online and will be open to all pre-registered participants. The practical part of the course will take place at the Institute of Zoology and Biomedical Research at Jagiellonian University in Krakow (ul. Gronostajowa 9, 30-387 Krakow, Poland), and will be available to participants who are selected based on an online questionnaire and cover letter. The number of participants for the hands-on part is strictly limited.
| Block title |
|---|
| 1. Fundamentals of CRISPR/Cas9 system |
| 2. Knock-out strategies & genotyping |
| 3. Knock-in strategies & genotyping |
| 4. Advanced editing technologies & genotyping |
| 5. gRNA design (online) & workflow overview |
| 6. Q&A |
| Block title |
|---|
| 1. CRISPR/Cas9 - preparation and injections |
| 2. Laboratory and zebrafish husbandry visit |
| 3. PCR setup for knock-out and/or knock-in genotyping |
| 4. Day 2 summary and Q&A |
| Block title |
|---|
| 1. Embryo assessment & phenotype analysis |
| 2. PCR results & genotyping interpretation |
| 3. Troubleshooting session |
| 4. Open Q&A & individual consultations |
| 4. Day 3 summary and course evaluation |
Anna Sarosiak
University Center for Women's and Newborn Health
Medical University of Warsaw, Poland
anna.sarosiak@gmail.com
Niedharsan Pooranachandran
Jagiellonian University
niedharsan.pooranachandran@uj.edu.pl
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