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2nd workshop of the Polish Zebrafish Society –principles of zebrafish model in toxicology
and behavioural studies
Lublin 30.11-01.12.2017 

Program
Lecturers
and practical parts tutors
Sponsors

Program


DAY I (30.11.2017)

8.30-9.00 Registration
9.00-9.30 Welcome and Introduction to Practical Part
9.30-13.30 Practicals - part I– coordinator: Piotr Podlasz (University of Warmia and Mazury in Olsztyn)
13.30-14.30 Lunch

LECTURES (open to public)
15.00-15.30 Introduction to zebrafish – Przemko Tylżanowski (Medical University of Lublin /University of Leuven)
15.30-16.00 Legal aspects (Lecture in Polish)- Mirosław Łańcut (Medical University of Lublin)
16.00-16.30 The Zebrafish model: 3R in husbandry and laboratory practice (International Institute of Molecular and Cell Biology in Warsaw)
16.30-16.45 Coffee break
16.45- 17.15 Alternative model - zebrafish in toxicology and neurotoxicology - Róbert Kovács (Animalab, Hungary)
17.15-17.35 Complete solution for high-throughput zebrafish larvae tracking - Patrick H. Zimmerman, (Noldus Information Technology, Wageningen, The Netherlands).
17.35-17.55 Aquaneering - zebrafish housing - Róbert Kovács (Animalab, Hungary)
18.00 – 20.00 Poster session/ Wine & cheese party




DAY II (01.12.2017)

9.00-12.30 Practicals - part II 12.30-13.30 Lunch & Discussion
13.30-14.50 Practicals - part III
14.50-15.00 Closing remarks and departure 

Practical parts schedule

DAY I

TimeGroup IGroup IIGroup IIIGroup IV
09.30– 10.30Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)
Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
Automated observation and tracking of larval zebrafish
(Patrick Zimmerman)
Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
10.30- 11.30Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)
Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
Automated observation and tracking of larval zebrafish
(Patrick Zimmerman)
11.30-12.30Automated observation and tracking of larval zebrafish
(Patrick Zimmerman)

Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)
Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
12.30-13.30Microinjections- Part I
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
Automated observation and tracking of larval zebrafish
(Patrick Zimmerman)

Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)

DAY II

TimeGroup IGroup IIGroup IIIGroup IV
9.00-9.50Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica)

Dissection of organs from adult zebrafish (Anna Jakimiuk)Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
9.50-10.40Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica)

Dissection of organs from adult zebrafish (Anna Jakimiuk)Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)
10.40-11.30Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica)

Dissection of organs from adult zebrafish (Anna Jakimiuk)
11.30-12.20Dissection of organs from adult zebrafish (Anna Jakimiuk)Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)Microinjections - Part II
(Przemko Tylżanowki, Aneta Chełstowska-Wejman, Małgorzata Wiweger)
Lithium treatment to disrupt Wnt signaling -Part II
(Natalia Kasica)

12.30-13.30Lunch & DiscussionLunch & DiscussionLunch & DiscussionLunch & Discussion
13.30-15.50Time for repetition of - selected issuesTime for repetition of - selected issuesTime for repetition of - selected issuesTime for repetition of - selected issues

Lecturers

Przemko Tylzanowski
Medical University of Lublin, Poland 
University of Leuven, Belgium
przemko@kuleuven.be 

Mobirise

Mirosław Łańcut
Medical University of Lublin, Poland
miroslawlancut@umlub.pl 

Mobirise

Małgorzata Wiweger
International Institute of Molecular and Cell Biology
in Warsaw, Poland  
mwiweger@iimcb.gov.pl
 

Mobirise

                         Róbert Kovács       
Animalab, Hungary        
 

Mobirise

                        Patrick Zimmerman   
Noldus Information Technology, Wageningen,
The Netherlands
                          
 

Mobirise

Practical parts tutors

Practical parts coordinator

Piotr Podlasz
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland 
piotr.podlasz@uwm.edu.pl
 

Mobirise

Anna Jakimiuk
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland  
annajakimiuk@wp.pl
 

Mobirise

Natalia Kasica-Jarosz 
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn
natalia_kasica@wp.pl 

Mobirise

Aneta Chełstowska-Wejman
Eppendorf 

Mobirise

Maciej Czerwiec                                                           
Sławomir Kowalczyk                                                        
Zeiss                                                
                                                       

Agnieszka Jakubaszek
Medical University of Lublin, Poland 
 

Mobirise

Anna Sobolewska
Medical University of Lublin, Poland 
 

Mobirise

Sponsors

We thank our generous sponsor

Address

Kazimierza Jaczewskiego 8 str                     
Lublin, 20-090

Contacts

Email: zebrafish.pl@gmail.com

Links


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