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1st PTZ workshop – principles of zebrafish model in toxicology and behavioural studies
Lublin, 20-21 April 2017

Program
Lecturers
and practical parts tutors
Sponsors

Program


DAY I (20.04.2017):

8.30-9.00 Registration
9.00-9.30 Welcome and Introduction to Practical Part
9.30-12.30 Practical part I – coordinator: Piotr Podlasz (University of Warmia and Mazury in Olsztyn) /Coffee break when needed
12.30-13.30 Sandwich lunch
13.30-14.15 Introduction to zebrafish - Przemko Tylzanowski (Medical University of Lublin /University of Leuven)
14.15-15.15 Legal aspects (Lecture in Polish)- Mirosław Łańcut (Medical University of Lublin)
15.15-15.45 Coffee break
15.45-16.30 Zebrafish husbandry, breeding and health – Małgorzata Wiweger (International Institute of Molecular and Cell Biology in Warsaw)
16.30- 17.15 Zebrafish model in toxicology - Camila Esguerra (Biotechnology Centre of Oslo, University of Oslo) (to be confirmed)
17.15-17.35 Anima IT (Techniplast)
17.35-17.55 Pros of lab husbandry of fish. -Tomasz Konicki Pentair/ Aquatic Habitats)
17.55 – 20.00 Poster session/ Wine & cheese party

DAY II (21.04.2017):


9.00-12.30 Practical part II (Coffee break when needed)
12.30-13.30 Lunch & Discussion
13.30-14.50 Practical part III (Coffee break when needed)
14.50-15.00 Final conclusions and departure 

Practical parts schedule

DAY I

TimeGroup IGroup IIGroup IIIGroup IV
09.30– 10.30Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)
Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot)
Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
10.30- 11.30Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)
Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot)
11.30-12.30Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot)
Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)
Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
12.30-13.30Microinjections- Part I
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
Automated observation and tracking of larval and adult zebrafish
(Antoine Barbot)
Visualization of IF staining/transgenic ZF (Piotr Podlasz, Maciej Czerwiec,
Sławomir Kowalczyk)
Lithium treatment to disrupt Wnt signaling- Part I
(Natalia Kasica)

DAY II

TimeGroup IGroup IIGroup IIIGroup IV
9.00-9.50Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk)
Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica)
Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
9.50-10.40Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk)
Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica)
Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)
10.40-11.30Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk)
Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica)
11.30-12.20Chemical screening for hair cell loss and protection - Part I
(Natalia Kasica)
Visit in the zebrafish facility (Agnieszka Jakubaszek, Anna Sobolewska)Microinjections - Part II
(Natalia Nowik, Przemko Tylżanowki, Aneta Chełstowska-Wejman)
Lithium treatment to disrupt Wnt signaling -Part II
(Anna Jakimiuk)
12.30-13.30Lunch & DiscussionLunch & DiscussionLunch & DiscussionLunch & Discussion
13.30-13.50Time for repetition of - selected issuesChemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec)
Time for repetition of - selected issuesDissection of organs from adult zebrafish (Anna Jakimiuk)
13.30-13.50Dissection of organs from adult zebrafish (Anna Jakimiuk)Time for repetition of - selected issuesChemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec)
Time for repetition of - selected issues
13.30-13.50Time for repetition of - selected issuesDissection of organs from adult zebrafish (Anna Jakimiuk)Time for repetition of - selected issuesChemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec)
13.30-13.50Chemical screening for hair cell loss and protection - Part II
(Natalia Kasica, Piotr Podlasz, Maciej Czerwiec)
Time for repetition of - selected issuesDissection of organs from adult zebrafish (Anna Jakimiuk)Time for repetition of - selected issues

Lecturers

Przemko Tylzanowski
Medical University of Lublin, Poland 
University of Leuven, Belgium
przemko@kuleuven.be 

Mobirise

Mirosław Łańcut
Medical University of Lublin, Poland
miroslawlancut@umlub.pl 

Mobirise

Małgorzata Wiweger
International Institute of Molecular and Cell Biology
in Warsaw, Poland  
mwiweger@iimcb.gov.pl
 

Mobirise

Camila Esguerra
Biotechnology Centre of Oslo, University of Oslo, Norway 
c.v.esguerra@biotek.uio.no
 

Mobirise
Tommaso Sala                                                                  
Tecniplast                                                         
                
Tomasz Konicki                                                                   
 Labtek/Pentair/Aquatic Habitats                                                         

Antoine Barbot                                                                  
 ViewPoint                                                        

Practical parts tutors

Practical parts coordinator

Piotr Podlasz
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland 
piotr.podlasz@uwm.edu.pl
 

Mobirise

Anna Jakimiuk
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland  
annajakimiuk@wp.pl
 

Mobirise

Natalia Kasica-Jarosz 
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn
natalia_kasica@wp.pl 

Mobirise

Natalia Nowik
Faculty of Veterinary Medicine
University of Warmia and Mazury in Olsztyn, Poland
natalia.nowik@uwm.edu.pl
Institute of Biology, Leiden University, The Netherlands
n.nowik@umail.leidenuniv.nl 

 

Mobirise

Aneta Chełstowska-Wejman
Eppendorf 

Mobirise

Maciej Czerwiec                                                           
Sławomir Kowalczyk                                                        
Zeiss                                                
                                                       

Agnieszka Jakubaszek
Medical University of Lublin, Poland 
 

Mobirise

Anna Sobolewska
Medical University of Lublin, Poland 
 

Mobirise

Sponsors

We thank our generous sponsor

Address

Kazimierza Jaczewskiego 8 str                     
Lublin, 20-090

Contacts

Email: zebrafish.pl@gmail.com

Links


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